Chromatography column

Chromatography column

Wedge column
Image

Wedge column

1) The flow rate is faster, the chromatographic efficiency is increased by more than 4 times, and the process development time is greatly shortened
2) Lower column back pressure, the wedge column can use the 15μm particle size or even below resin which can not be used in current process because of the pressure limited
3) Easy to scale up, better process scale up consistency, and smaller footprint after scale up
4) Better column efficiency and separation trend
5) Prevent concentration dependent precipitation and improve the yield
6) Easy to use, no need to disassemble to achieve resin packing and unpacking

  • Description

  • Brand

  • Reference

Overview：
The Wedge column is a scale down model of the larger radial column, similar to cutting a cake. The large radial column is the cake, and the Wedge column is a small piece cut out. As shown in the picture below:

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The Wedge column is shaped like a wedge, and the upper surface of the column head is significantly larger than the lower surface, allowing easy linear amplification from laboratory-scale columns to large-scale radial-flow columns.

Product features:
1) The flow rate is faster, the chromatographic efficiency is increased by more than 4 times, and the process development time is greatly shortened
2) Lower column back pressure, the wedge column can use the 15μm particle size or even below resin which can not be used in current process because of the pressure limited
3) Easy to scale up, better process scale up consistency, and smaller footprint after scale up
4) Better column efficiency and separation trend
5) Prevent concentration dependent precipitation and improve the yield
6) Easy to use, no need to disassemble to achieve resin packing and unpacking

Application Case:
The sample volume is scale up from 20ml to 600ml, and the scale up is 30 times, that is, only the column volume is scale up in equal proportion (400ml to 12L), and the flow rate is increased in equal proportion (from 95ml/min to 2850ml/ml), and the consistent purification effect is easily achieved

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Order List：

Name

Application

Cat.no

Volume

Bed depth

Diagram

Wedge acrylic column

Lab scale

WA-0005-10

5mL

10cm

image.png

WA-0010-10

10mL

10cm

WA-0020-10

20mL

10cm

WA-0050-10

50mL

10cm

WA-0100-10

100mL

10cm

WA-0250-10

250mL

10cm

WA-0500-10

500mL

10cm

WA-1000-10

1L

10cm

WA-0020-15

20mL

15cm

WA-0100-15

100mL

15cm

WA-0250-15

250mL

15cm

WA-0500-15

500mL

15cm

WA-1000-15

1L

15cm

Wedge

stainless

column

WS-0100-10

100mL

10cm

image.png

WS-0250-10

250mL

10cm

WS-0500-10

500mL

10cm

WS-1000-10

1000mL

10cm

WS-0100-16

100mL

15cm

WS-0250-16

250mL

15cm

WS-1000-16

1000mL

15cm

Based in California, Sepragen Corporation provides the biotechnology and nutraceutical industries with breakthrough products and processes to meet their process development and manufacturing needs.

Sepragen Corporation was founded in 1985 by Vinit Saxena, the inventor of radial flow chromatography technology. The company currently has 16 core patented technologies. Its main products include chromatography columns and their filling systems, chromatography systems, chromatography resins, bioreactors and Other accessories etc. Since its establishment, the company has cooperated with major well-known companies in North America, Europe, Australia and Asia and has received widespread praise. 


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