Chromatography column

Chromatography column

Radial Column
Image

Radial Column

1) The flow rate is faster, the chromatographic efficiency is increased by more than 4 times, and the process development time is greatly shortened
2) Lower column back pressure, the wedge column can use the 15μm particle size or even below resin which can not be used in current process because of the pressure limited
3) Easy to scale up, better process scale up consistency, and smaller footprint after scale up
4) Better column efficiency and separation trend
5) Prevent concentration dependent precipitation and improve the yield
6) Easy to use, no need to disassemble to achieve resin packing and unpacking

  • Description

  • Brand

  • Reference

The radial flow chromatography technique is different from the traditional axial flow chromatography technique. In the radial column , the mobile phase carries the sample move along the radial direction. In a radial-flow column, the mobile phase and the sample can flow from the outer annulus of the column to the center of the column.

The radial column is composed of two annulus (see Figure.1). The area of the outer annulus is significantly larger than that of the inner annulus. A cavity is formed between the inner and outer annulus. The sample permeates through the entire outer annulus to the inner annulus, and finally the sample gathers with the inner annulus and exits through the lower port.

图片5

Pack and unpack of radial column
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Product features:
1) The flow rate is faster, the chromatographic efficiency is increased by more than 4 times, and the process development time is greatly shortened
2) Lower column back pressure, the wedge column can use the 15μm particle size or even below resin which can not be used in current process because of the pressure limited
3) Easy to scale up, better process scale up consistency, and smaller footprint after scale up
4) Better column efficiency and separation trend
5) Prevent concentration dependent precipitation and improve the yield
6) Easy to use, no need to disassemble to achieve resin packing and unpacking


Application Case:
1) Pressure comparison of ovalbumin separation system between radial chromatographic column and traditional axial column
2
2) Comparison of protease purification expressed by E. coli isolated by radial chromatographic column and traditional axial column

1

Order List：

Name

Application

Cat.no

Volume

Bed depth

Diagram

acrylic pre-pack column

Lab scale

PP-0050-00

50mL

3.5cm

image.png

PP-0100-00

100mL

3.5cm

PP-0250-00

250mL

3.5cm

PP-0350-00

350mL

3.5cm

PP-0500-00

500mL

3.5cm

PP-1000-00

1L

3.5cm

Superflo column

Lab scale

10-0050-00

50mL

3.5cm

image.png

10-0100-00

100mL

3.5cm

10-0250-00

250mL

3.5cm

10-0500-00

500mL

3.5cm

10-1000-00

1L

3.5cm

10-1500-00

1.5L

3.5cm

Superflo column

Pilot scale

11-002K-00

2L

5cm

image.png

11-003K-00

3L

5cm

11-005K-00

5L

5cm

11-07.5K-00

7.5L

5cm

Superflo column

Production scale

11-010L-00

10L

10cm

image.png

11-020L-00

20L

10cm

11-030L-00

30L

10cm

11-040L-00

40L

10cm

11-050L-00

50L

10cm

11-060L-00

60L

10cm

11-070L-00

70L

10cm

Superflo stainless column

Lab scale

13-0100-00

100mL

3.5cm

image.png

13-0250-00

250mL

3.5cm

13-0500-00

500mL

3.5cm

13-1000-00

1L

3.5cm

13-1500-00

1.5L

3.5cm

Superflo stainless column

Pilot scale

13-005K-00

5L

5cm

image.png

13-010K-00

10L

5cm

13-015K-00

15L

5cm

Superflo stainless column

Production scale

13-010L-00

10L

10cm

image.png

13-015L-00

15L

10cm

13-020L-00

20L

10cm

13-025L-00

25L

10cm

13-030L-00

30L

10cm

13-040L-00

40L

10cm

13-050L-00

50L

10cm

13-060L-00

60L

10cm

13-070L-00

70L

10cm

13-080L-00

80L

10cm

13-090L-00

90L

10cm

13-100L-00

100L

10cm

custom-made

Production scale

custom-made 110-400L Superflo column

 


Based in California, Sepragen Corporation provides the biotechnology and nutraceutical industries with breakthrough products and processes to meet their process development and manufacturing needs.

Sepragen Corporation was founded in 1985 by Vinit Saxena, the inventor of radial flow chromatography technology. The company currently has 16 core patented technologies. Its main products include chromatography columns and their filling systems, chromatography systems, chromatography resins, bioreactors and Other accessories etc. Since its establishment, the company has cooperated with major well-known companies in North America, Europe, Australia and Asia and has received widespread praise. 


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