Gene Editing
Gene Editing
Super Electro Cell Fusion Generator ECFG21
- High cell fusion efficiency
- High cell viability
- Constant AC voltage and ultra-short AC/DC switching time
- The new electrofusion program realizes the design of voltage attenuation and polarity alternating pulse (+/-), and the fusion parameters are visible and adjustable
- Especially suitable for the preparation of monoclonal antibodies, animal cloning and other fields
Super Electro Cell Fusion Generator ECFG21, the preparation efficiency of monoclonal antibody is much higher than that of PEG method, and it does not depend on the operator. Guaranteed batch stability. The novel three-step electrofusion procedure, based on the conventional electrofusion procedure, with the patented voltage attenuation and polarity alternating pulse (+/-) design, can obtain a higher fusion efficiency and improve cell viability while obtaining a longer repair time after fusion.
- Step 1：Cells are alignment in alternating current mode
The constant alternating current wave aligns the cells together in a fast two-dimensional electrophoresis.
- Step 2：cells are fused in direct current mode
AC/DC switching in a very short time, voltage decay design, can generate forward pulses (+) or polar alternating pulses (+/-). The cells are fused with little damage, and the experimental results show that the pulses with alternating polarity can improve the fusion rate compared with the positive pulses alone.
- Step 3：Cells are repaired in alternating current mode
Longer cell repair time, optional sine wave with voltage attenuation, stabilize the fusion state of heterozygous cells, promote cell damage repair, and improve survival rate.
Key features:
- High cell fusion efficiency
- High cell viability
- Constant AC voltage and ultra-short AC/DC switching time
- The new electrofusion program realizes the design of voltage attenuation and polarity alternating pulse (+/-), and the fusion parameters are visible and adjustable
- Especially suitable for the preparation of monoclonal antibodies, animal cloning and other fields
Applications:
Electroporation
Electrofusion
- Splenocytes are fused with tumor cells for the preparation of monoclonal antibodies
- Plant protoplast fusion
- Fusion of mold , yeast
Animal cloning
- Nuclear transfer, somatic cell nuclear transplantation, somatic cell transfer, somatic cell fusion with egg cell
- 2-cell blastomeres are electrofused to make tetraploid embryos
- Activation of mature oocytes in vitro
- Applicable to a wide range of species, rats, pigs, cats, dogs, wolves, etc
Application example: Miniature pig clone
The production of NEPA21 series electroporation instrument, ECFG21 high-efficiency cell electrofusion and electroporation instrument and PicoPipet single-cell separation and extraction system has been well-known in the research field and has been cited by hundreds of literatures, including high-level journal articles, such as Nature, Cell, PNAS, Genes & Dvelopment, etc.
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