• Automatic staining
technology
• Analyze 24 samples in less than 13 minutes
• Only 30 μL of cell sample
required
• Calibration, maintenance
and service free
• Customizable PDF reports
• 21 CFR part11 compliant
Countleader
FL 2000 cell counter is a high-throughput automated system for cell counting
analysis using the FL24-plate with pre-coated dyes and analyzing 24 samples in
13 minutes to meet the needs of customers for multi-samples analysis.
Key Features & Benefits
• Automatic staining
technology
• Analyze 24 samples in less
than 13 minutes
Total cell, live
cell, dead cell count
Viability
Cell diameter
Growth curve
% of cells in
clumps
• Only 30 μL of cell sample
required
• Calibration, maintenance
and service free
• Customizable PDF reports
• 21 CFR part11 compliant
Product Details
Automatic staining technology
The
FL24-plate is designed to be coated with AO/PI dyes in the microfluidic
channel, once the cell samples are loaded, the cell samples are automatically
aspirated and flow through the microfluidic channel, stained by the coated
AO/PI automatically and finally pushed into the detection chamber. In the
chamber, the stained samples are excited and analyzed, all the cell counting
data are displayed in the software.
The FL24-plate estimates the error by manual, resulting in accurate data, makes your work more efficient and convenient!
The
inside of the microfluidic channel of FL24-Plate is coated with Acridine orange
and PI, staining the entire population of cells and non-viable cells
respectively. The volume of each FL24-Plate has been calibrated to provide high
precision cell counting data.
Easy operation process
Sample Loading directly!
Loading
30μL of sample into the FL24-plate plate with Multi-channel pipette, no
pretreatment of the samples is required.
Load and press “Run”
The
FL24-plate with samples is loaded into the FL2000 and Press Run.
Automatic staining and analysis
Samples are stained automatically as the samples flow through the microfluidic channel coated with AO/PI. The analysis process is fully automated, accurate cell density, viability and fluorescence images of 24 cell samples are presented in less than 13 minutes.
Powerful and flexible software
The software is powerful, friendly and flexible, detection channels can
be selected according to your sample numbers.
• Typan blue and AO/PI modes
are available
• Larger analysis volume,
Less statistical errors
• The data can be searched
by experiment name, time, and sample ID
• Separate sample ID
facilitate results browsing and searching
• Dilution Factor is
compensated automatically
• Get cell growth curves to understand the trend of cell growth
Accurate Data
Automatic staining and analysis make the results accurate, without the error from the staining of samples by manual. With 1.5X magnification, the area of analysis of field of view is about 4.7 * 3.1m2, which is almost 12 times of that in the Hemocytometer method. The number of cells detected in a single sample can reach tens of thousands, effectively reducing counting errors.
Intuitive
and accurate data with total cell concentration, live and non-viable cell
concentration, cell viability, cell diameter and Aggregated cell ratio can be
obtained fastly.
Multiple
results are represented in the software. The data can be represented in
histograms, fluorescent scatter plots and result boxes in the Analysis Manager
Plot.
All the data can be exported in PDF, Excel, TIFF format.
Polygons and markers can be drawn in the plots and are used for gating and counting of subpopulations. The percentage of cells with different diameter can be achieved to know the cell status such as stem cell differentiation, virus infection efficiency.
Tracking
and detecting information of continuously cultured cell samples can be plotted
as a cell growth curve.
√Concentration
curve
√Viability
curve
It is
easy to track the cell growth state and trends of cells, which is conducive to
the optimization of cell culture technology.
21 CFR Part 11 Compliant
Countleader
FL 2000 has strict 3-levels user management and authority, meet the 21 CFR Part
11 requirements of GMP. Modules have the following functions:
•
User login with passwords
•
User assigned permissions
•
Audit trail
•
Log files
• Electronic records and signatures
IQ/OQ/PQ Verification
Countleader FL 2000 provides 3Q verification service with a variety of concentration beads and verification documents to facilitate the later declaration work.
Compatible with various consumables
In addition to the FL24-plate coated with AO/PI dyes, Countleader FL2000 is compatible with other different consumables, empty 24-channel FL24-plate without coated dye and 8-channel FL8-slides, manual staining can also be used for Trypan blue stain counting in Countleader FL 2000.
Product Applications
In the
cell line development and process development, many samples are required for
cell counting, the daily cell count workload is large. Countleader FL 2000
provide the solution with high throughput, low sample consumption, fast and
automatic staining and analysis. Countleader FL 2000 can detect 24 samples in
less 13 minutes, improves cell counting efficiency greatly!
•
CLD department in Antibody and CGT field
•
PBMC, Car-T in cell therapy
•
Stem Cells
•
Cancer Cells
• and more