AbioBundle Series Glass Bioreactors

With nearly 20 years of experience in the biopharmceutical industry and a dedication to craft high-end bioreactors, Applitech proudly presents the AbioBundle Series Glass Bioreactors. Backed by a highly skilled R&D team of over 150 professionals, the AbioBundle line represents years of refinement and expertise. Designed with a deep understanding of user needs, AbioBundle M/E/P/D series bioreactors set new standards in process development. With AbioBundle, Applitech provides unparalleled support for your bioprocess development.

1.1 AbioBundle M-Series Bioreactors

The AbioBundle M Series Bioreactors feature a classic small-scale bioreactor design, enabling precise downscaling of laboratory-scale reactors while maintaining the same flexibility and adaptability. The system offers customizable configurations to meet diverse customer needs.

M-Control

Features:

• The working volume covers 50ml~2.7L.
• Embedded m-Control offers multi-functionality in a small footprint; customizable casing in multiple colors.
• Parallel cultivation with independent monitoring of each reactor.
• Up to 32 m-Control controllers form a network of parallel reactors.
• Control operations easily through web browsers or mobile devices (data can be stored and monitored without SCADA software).
• Data can be scaled up directly, minimizing media consumption and saving cost.
• 250mL/500mL small bioreactors use a Peltier-based temperature control system, eliminating the need for a chiller.
• Small footprint, less than 1m² for a 4-tank system.
• Online automatic detection of control parameters: pH, temperature, DO, agitation, level, foam, etc.
• Modular design allows easy adjustments to suit various applications and process control strategies.
• AiOpt algorithm for precise control of process parameters.
• Up to 4 variable-speed pumps, each with an independent Prime button.
• Up to 5 gas mass flow controllers (MFCs).
• Supports commonly used electrodes in the market and is compatible with smart electrodes.
• Total Gas Flow Control
• The aeration system includes 1 overlay and 2 sparger ports, with options for different sparger sizes and shapes.
• The agitation system features a servo motor and various impeller types, suitable for cell culture or microbial fermentation.
• Equipped with a unique breathing light to indicate system working status.
• Compatible with external devices such as cell density modules, fluorescent pH/DO electrodes, balances, etc.
• Comes standard with AbioPIMS Lite or AbioExpert Lite software.
• (Optional) Configurable to support vessel sizes up to 15L.
• (Optional) Enables remote monitoring capabilities.
• (Optional) Provides remote alarm notifications via SMS, phone calls, email or WeChat.
• (Optional) Advanced functionalities such as enhanced feeding strategies tailored to customer needs.

Size & Model

1.2 AbioBundle E-Series Bioreactors

The AbioBundle E Series Bioreactors are robust, flexible, and easy to operate, with options for upgrades and expansions. Ideal for mammalian cell culture and microbial fermentation R&D projects, they offer configurations for single, dual, or quad controls. Supporting both single-wall and jacketed vessels, the series meets the demands of complex processes and is suited for a wide range of applications. The dual/quad control modes allow for mixing and matching of vessels with different volumes and types, all operating independently.

E-Control

e Dual -Control

Features:

• Modular Design: Add or remove functional modules based on application requirements.

• No welded components on the head plate; all metal parts are made from 316L stainless steel. Non-contact areas have a mirror-polished finish, and contact areas are electro-polished for easy cleaning.

• Borosilicate Glass Vessels offer excellent resistance to shock and corrosion with smooth surfaces for easy cleaning.

• Available with single-control (e-Control), dual-control (eDual-Control, supports quad-control).

• 12" screen for single control and 13.3" for dual control; colorful touchscreen interface for intuitive operation.

• Built-in storage to ensure no data loss.

• Supports up to 23 operator accounts simultaneously with three-level password protection.

• eDual-Control features a web interface for easy bioreactor setup and control via a browser.

• (Optional) Biomass module enables real-time cell density measurement and feedback feeding operations.

• Agitation system: Equipped with a servo motor and mechanical seal stirrer; upgradeable to magnetic coupling.

• (Optional) Enhanced gas module allows switching between 1 overlay and 2 sparger pathways per gas.

• Each vessel supports up to 6 mass flow controllers (MFCs).

• Feeding control: Supports external balance integration for advanced feeding strategies via PID control.

• e-Control supports up to 4 constant-speed pumps, with optional variable-speed pumps. eDual-Control supports up to 4 variable-speed pumps per vessel, each with an independent mechanical Prime button.

• Total Gas Flow Control: Maintains constant total gas flow during culture to prevent excessive shear forces on cells.

• Comes standard with AbioPIMS Lite or AbioExpert Lite software.

• (Optional) Enables remote monitoring capabilities.

• (Optional) Provides remote alarm notifications via SMS, phone calls, email or WeChat.

• (Optional) Advanced functionalities such as enhanced feeding strategies tailored to customer needs.

Size & Model

P-Control

Features:

• The working volume covers 0.3~16L.

• Touchscreen HMI, intuitive and user-friendly interface for simplified operation.

• Siemens PLC platform.

• Standard Fieldbus I/O Design to failitate easy maintenance and functional expansion.

• Flexibility & Scalability, providing superior reliability and adaptability.

• Open plateform that supports integration with systems like Pi data recording.

• Compliant with ISAS88, GAMP5, cGMP, etc.

• Measurement and Control Parameters:

• pH, dissolved oxygen, temperature, foam, level, agitation.

• Up to 6 gas channels per vessel.

• Balance

• Up to 4 built-in variable-speed pumps per vessel

• Enables simultaneous control of multiple bioreactors with different sizes, making scale-up more efficient.

• Comes standard with AbioPIMS Lite or AbioExpert Lite software.

2. AppliSoftec Data Management Software

AbioPIMS/AbioExpert complies with GAMP5, 21 CFR Part 11, and ANSI/ISA-88.01 Batch Control System Standards, offering robust audit trails. The software facilitates real-time data acquisition of bioprocess parameters, storing data in database for historical data analysis and traceability, and empowering enhanced production, quality, and equipment management.

AbioPIMS/AbioExpert is compatible with OPC UA protocol. AbioExpert can integrate with external devices (such as Nova Biochemical Analyzer, Kaiser Raman Analyzer, and cell counters) for advanced process strategies.

AbioPIMS/AbioExpert has a RECIPE design functionality with a graphical programming interface. Users can implement complex process strategies without any programming expertise.

AbioPIMS/AbioExpert is compatible with Applikon my-Control/ez-Control/ez2-Control series bioreactors

AppliSoftec Bioprocess Information Management System

Access Management:

Password protection; customizable access/operation permissions; user group configuration

21 CFR Part 11:

Adheres to 21 CFR Part 11 standards, offering three security levels: Operator, Supervisor, and Administrator.

Provides event logging, audit trails, and electronic signature capabilities.

Configuration

With a variety of data acquisition port configuration, the system specifies the data acquisition channel and data import template file.

① Supports multi-device integration with connection to parameter configu rations and control strategies.

② Advanced alarm settings: high-high, high, low, low-low thresholds with customiz able alarm and response strategies.

③Flexible storage modes: periodic or event-based, with value transformations and display configurations.

OPC

Built on an OPC platform, the OPC Client ensures flexible device connectivity, enhancing control over critical process parameters.

UI

Intuitive configuration processes streamline usability and accelerate task execution.Navigation tools allow quick access to configuration, monitoring, trending, alarms, and sampling modules.

Data Monitoring & Management：

Comprehensive monitoring and historical data recording with periodic and event-driven save modes; ensures data integrity and security, enabling fast, robust, and searchable data management; supports exporting data and configuration information into customizable reports; includes extensive system logs, alarm histories, and traceable operational records.

CHART

The chart window provides robust visualization capabilities, allowing users to view all relevant process parameters and configure online trends as needed:

① Options for zooming, panning, stretching, clipping, and selecting time ranges. Users are also able to modify curve styles, choose to hide or display parameters, and add, edit, or delete individual charts.

②Compares and track process data across up to 8 historical batches simulta neously, aligned on a process timeline; synchronizes comparisons between current running data and historical data on the same timeline for comprehen sive analysis.

MONITOR

Quick switching to 24 monitoring units, allowing detailed visualization of all relevant information. Customizable monitoring module displays and navigation to menus like batch operations, alarms, recipes, real-time trends, and sampling.

CONTROL:

Capability for individual or batch parameter control.

Recipe Design:

Simple and intuitive recipe design guided by on-screen instructions. Allows advanced control strategy development, automatically adjusting setpoints based on time and cultivation conditions. Requires no programming skills; users can drag and drop logic blocks to create branches, loops, waits, jumps, or parallel executions.

Calculator Module:

Efficiently transforms data with calculation tools for key bioprocess parameters and indirect variables. Integrates seamlessly into recipe modules to enable logical connections and process automation.

Additional Information

Free Trial

All AppliSoftec software series come with a free trial for the first 60 days post-installation.

Software Updates

AppliSoftec software is continuously updated, and customers can freely upgrade to the corresponding versions.

Minimum System Requirements

Processor/Clock Rate: Multi-core processor, 2.5 GHz

RAM: 8 GB (16 GB recommended)

Hard Disk Space: 256 GB

Optical Drive: DVD-ROM drive

Network Adapter: 1+ LAN adapter, RJ-45, 100 Mbit

Graphics Card: DirectX 10, 1 GB RAM (recommended)

Screen Resolution: 1920 x 1080, 100% scaling

Display Monitor: 23.8-inch monitor

Operating System: Microsoft Windows 10 (64-bit)

Runtime Components: .NET Framework 4.0 / .NET Framework 5.0 / .NET Core 3.1

Other Software: Microsoft Excel (2013 or newer recommended) / SQL Server 2014 / MongoDB