Chromatography Column
Chromatography Column
Radial Column
1) The flow rate is faster, the chromatographic efficiency is increased by more than 4 times, and the process development time is greatly shortened
2) Lower column back pressure, the wedge column can use the 15μm particle size or even below resin which can not be used in current process because of the pressure limited
3) Easy to scale up, better process scale up consistency, and smaller footprint after scale up
4) Better column efficiency and separation trend
5) Prevent concentration dependent precipitation and improve the yield
6) Easy to use, no need to disassemble to achieve resin packing and unpacking
The radial flow chromatography technique is different from the traditional axial flow chromatography technique. In the radial column , the mobile phase carries the sample move along the radial direction. In a radial-flow column, the mobile phase and the sample can flow from the outer annulus of the column to the center of the column.
The radial column is composed of two annulus (see Figure.1). The area of the outer annulus is significantly larger than that of the inner annulus. A cavity is formed between the inner and outer annulus. The sample permeates through the entire outer annulus to the inner annulus, and finally the sample gathers with the inner annulus and exits through the lower port.
Pack and unpack of radial column
Product features:
1) The flow rate is faster, the chromatographic efficiency is increased by more than 4 times, and the process development time is greatly shortened
2) Lower column back pressure, the wedge column can use the 15μm particle size or even below resin which can not be used in current process because of the pressure limited
3) Easy to scale up, better process scale up consistency, and smaller footprint after scale up
4) Better column efficiency and separation trend
5) Prevent concentration dependent precipitation and improve the yield
6) Easy to use, no need to disassemble to achieve resin packing and unpacking
Application Case:
1) Pressure comparison of ovalbumin separation system between radial chromatographic column and traditional axial column
2) Comparison of protease purification expressed by E. coli isolated by radial chromatographic column and traditional axial column
|
Name |
Application |
Cat.no |
Volume |
Bed depth |
Diagram |
|
acrylic pre-pack column |
Lab scale |
PP-0050-00 |
50mL |
3.5cm |
|
|
PP-0100-00 |
100mL |
3.5cm |
|||
|
PP-0250-00 |
250mL |
3.5cm |
|||
|
PP-0350-00 |
350mL |
3.5cm |
|||
|
PP-0500-00 |
500mL |
3.5cm |
|||
|
PP-1000-00 |
1L |
3.5cm |
|||
|
Superflo
column |
Lab
scale |
10-0050-00 |
50mL |
3.5cm |
|
|
10-0100-00 |
100mL |
3.5cm |
|||
|
10-0250-00 |
250mL |
3.5cm |
|||
|
10-0500-00 |
500mL |
3.5cm |
|||
|
10-1000-00 |
1L |
3.5cm |
|||
|
10-1500-00 |
1.5L |
3.5cm |
|||
|
Superflo
column |
Pilot
scale |
11-002K-00 |
2L |
5cm |
|
|
11-003K-00 |
3L |
5cm |
|||
|
11-005K-00 |
5L |
5cm |
|||
|
11-07.5K-00 |
7.5L |
5cm |
|||
|
Superflo
column |
Production
scale |
11-010L-00 |
10L |
10cm |
|
|
11-020L-00 |
20L |
10cm |
|||
|
11-030L-00 |
30L |
10cm |
|||
|
11-040L-00 |
40L |
10cm |
|||
|
11-050L-00 |
50L |
10cm |
|||
|
11-060L-00 |
60L |
10cm |
|||
|
11-070L-00 |
70L |
10cm |
|||
|
Superflo
stainless column |
Lab
scale |
13-0100-00 |
100mL |
3.5cm |
|
|
13-0250-00 |
250mL |
3.5cm |
|||
|
13-0500-00 |
500mL |
3.5cm |
|||
|
13-1000-00 |
1L |
3.5cm |
|||
|
13-1500-00 |
1.5L |
3.5cm |
|||
|
Superflo
stainless column |
Pilot scale |
13-005K-00 |
5L |
5cm |
|
|
13-010K-00 |
10L |
5cm |
|||
|
13-015K-00 |
15L |
5cm |
|||
|
Superflo
stainless column |
Production
scale |
13-010L-00 |
10L |
10cm |
|
|
13-015L-00 |
15L |
10cm |
|||
|
13-020L-00 |
20L |
10cm |
|||
|
13-025L-00 |
25L |
10cm |
|||
|
13-030L-00 |
30L |
10cm |
|||
|
13-040L-00 |
40L |
10cm |
|||
|
13-050L-00 |
50L |
10cm |
|||
|
13-060L-00 |
60L |
10cm |
|||
|
13-070L-00 |
70L |
10cm |
|||
|
13-080L-00 |
80L |
10cm |
|||
|
13-090L-00 |
90L |
10cm |
|||
|
13-100L-00 |
100L |
10cm |
|||
|
custom-made |
Production scale |
custom-made 110-400L Superflo column |
|||
Latest posts
Based in California, Sepragen Corporation provides the biotechnology and nutraceutical industries with breakthrough products and processes to meet their process development and manufacturing needs.
Sepragen Corporation was founded in 1985 by Vinit Saxena, the inventor of radial flow chromatography technology. The company currently has 16 core patented technologies. Its main products include chromatography columns and their filling systems, chromatography systems, chromatography resins, bioreactors and Other accessories etc. Since its establishment, the company has cooperated with major well-known companies in North America, Europe, Australia and Asia and has received widespread praise.
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