Single-use disposable sterile consumables

Cross-contamination can be avoided by using single-use, aseptic filling consumables. The irradiated filling tube assembly is packaged in a double-bagged aseptic bag, providing reassurance on product integrity. The left side of the filling tube can be connected to shake tube, shake bottle, sterile wave bag, etc.

Easy to operate

The installation of the filling tube set is easy, just load and fix on the rack. Remove the needle sleeves which is used to ensure needle sterility for cell filling.

Fast, automated filling with great consistency

Compared with manual processing, the automated filling system can remove the error from manual, fill the cryovials quickly and simply, and ~1000 cryovials can be easily filled in 30 minutes.

The volume accuracy was tested by filling 500 μL, 1000 μL and 4000 μL respectively, the data shows volume accuracy error was <5%.

3 different concentrations of cells were filled into 24 cryovials, and the cell viability deviation was <2%, and the viable cell concentration deviation was <5%. The AbioFill V100 has excellent consistency, which is more conducive to the establishment of higher quality and consistent cell banks.

Easy and intuitive software operation

▪ Customized Filling models: Select the appropriate module, cryovial rack according to the cryovial type

▪ Multiple operation modes:

• "Decap – Filling – recap" mode

• "Filling Only" mode

• "Decap only" mode

• "Recap Only" mode

▪ Filling flow rate: fast filling, 350-800 μL/s can be selected, ~1000 cryovials can be easily filled in 30 minutes.

▪ Filling volume range is from 250 μL to 5 mL with good consistency.

▪ Number of Fillling columns: the cryovials can be loaded (4, 8, 12, 16, 20, 24 vials) in order, and columns can be set accordingly

▪ Cumulative quantity and capacity: The software tracks the number and total capacity of Filled cryovials in real time.

Audit trail, User Management

AbioFill V100 is GMP compatible with audit trail and multi-level authority management functions:

• Account and password login

• Multi-level user management

• Audit trails

UV sterilization

Customized UV sterilization lamp is optional, and it is controlled by software to sterilize the filling module and the rack transfer unit.

Compatible with Various Cryovials

• AbioFill V100 system is flexible and compatible with various Cryovials and the relevant accessories can be individually customized

• Filling volumes range: 250 μL-5 mL

• AbioFill V100 comes with accessories including cryovial racks and decap/recap module to accommodate the cryovial types selected.

• More brands of cryovial accessories can be customized.

Applications

Filling of high-yield and stable tertiary cell bank

The establishment of a three-level cell bank can provide qualified, of the same quality, and continuously stable passable cells for the production of biological products. The filling instrument AbioFill V100 can easily achieve large-scale cell filling through automated operation.

Strains, plasmids, viral vector banks establishment

Plasmids are important tools for cell, gene and vaccine therapy, and it is important to establish a stable tertiary strain bank for the production of plasmids. In the field of gene and cell therapy, transient technology is used at present, which requires a large number of GMP grade plasmids. In the research of therapeutic drugs, vaccines, etc., plasmids can be injected into the human body as the end product to express the target genes. AbioFill V100 enables efficient aseptic filling of tertiary strain and plasmid bank.

Filling for biological agents such as cytokines, virus solutions, etc

Biological agents have strict temperature requirements for preservation due to their biological activity, so the filling process is particularly important. The agents are usually packed in small, volume-matched freezing containers, such as cryovials with different size.