Constant Systems is a British company specializing in the production of high-pressure cell and is a member of the Score Group PLC group. Constant Systems has been committed to the development and production of high-pressure disruptors, providing users with high-quality and stable performance disruptors. Through its global marketing network, its products are sold to various parts of the world, including North America, Europe, the Middle East, and Asia, providing high-performance high-pressure disruptors and excellent services to pharmaceutical and biotechnology companies, universities, and research institutions worldwide.
Constant Brand Introduction:
Constant Systems is a British company specializing in the production of high-pressure cell
and is a member of the Score Group PLC group. Constant Systems has been committed to the development and production of high-pressure disruptors, providing users with high-quality and stable performance disruptors. Through its global marketing network, its products are sold to various parts of the world, including North America, Europe, the Middle East, and Asia, providing high-performance high-pressure disruptors and excellent services to pharmaceutical and biotechnology companies, universities, and research institutions worldwide.
Overview of Constant High Pressure Cell Disruptor:
The Constant Systems high-pressure cell disruptor can achieve a maximum pressure of 40000 PSI (2700 bar) and can disrupt microorganisms, animal cells, plant cells, tissues, and homogenization. The Constant Systems high-pressure disruptor has several models including One Shot, TS series, B series, C series, D series, E series, and F series. All models of disruptors can achieve very good disrupting effects after one disrupting. The Constant Systems high-pressure disruptor can conveniently perform CIP (in place cleaning) and SIP (in place disinfection) to meet GMP standards.
Specific
model of Constant cell disruptor:
Constant High Pressure Cell Disruptor
Domestic assembly models |
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Product
range |
Max Pressure Rating PSI/BAR |
FLOW RATE |
Oneshot |
40KPSI/2700Bar |
0.5-8ml |
TS0.75KW |
40KPSI/2700Bar |
40ml/min(2.4L/hr) |
TS1.1kw |
40KPSI/2700Bar |
100ml/min(6L/hr) |
Constant High Pressure Cell Disruptor Original imported models from the UK |
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Product range |
Max
Pressure Rating PSI/BAR |
FLOW
RATE |
OS |
40KPSI/2700Bar |
0.5-8ml |
MC |
40KPSI/2700Ba |
1-40mL |
CF1 |
40KPSI/2700Ba |
100ml/min(6L/hr) |
CF2 |
40KPSI/2700Bar |
400ml/min(24L/hr) |
HFR |
40KPSI/2700Bar |
150L/hr |
The
principle of cell fragmentation by a Constant cell disruptor
The Constant Systems high-pressure cell disruptor
is specifically developed for biotechnology users, taking into account the
characteristics of biological samples in design. It has unique designs for
sample cooling, CIP, and SIP. The principle of the Constant Systems
high-pressure disruptor is to use high-pressure extrusion of samples to pass
through a fixed small nozzle at high speed, and then collide with the impact
target to break the cells.
The high-pressure piston moves downwards, and
due to gravity, the sample is sucked into the high-pressure sleeve
Once the sample enters the high-pressure chamber, the
piston accelerates at high speed to close the inlet valve and allows the sample
to pass through the fixed nozzle at high speed. The sample pressure can reach
40kpsi (2700 bar), and the sample quickly transitions from the high-pressure
zone to the low-pressure zone, causing cell fragmentation
The sample hits the impact target and flows through the cooling surface. The cooled sample flows out of the outlet due to gravity and enters the lower cycle
Temperature
control
By using a cooling jacket to cool the sample
during the disrupting process, the temperature of the disrupted sample can be
well controlled, thereby avoiding the deactivation of active substances.
Reproducibility
The Constant Systems fluid control system
precisely controls the disrupting pressure, ensuring the reproducibility of the
instrument and good disrupting effect.
Technical
characteristics of Constant cell fragmentation equipment
Constant Systems can generate a pressure of up to 40Kpsi (2700 bar), with good disrupting effect. One disrupting can achieve very high disrupting efficiency, avoiding multiple disrupting that may cause fine fragments and denaturation and deactivation of active substances.
Constant Systems disrupting head has a cooling jacket design, which can effectively control the temperature of disrupted samples; Enable the sample to be cooled in a timely manner during the disrupting process, thereby avoiding the deactivation of active substances due to temperature rise in the disrupted sample; In addition, the sample storage tank can also be equipped with a jacket for sample pre cooling.
Constant Systems adopts a fixed nozzle design, which has good stability; Overcoming the disadvantages of valve design that relies on regulating valves to regulate disrupting pressure, such as poor pressure stability, poor reproducibility, and easy valve failure during the disrupting process.
Constant Systems uses hydraulic systems to generate high pressure, with stable pressure and good reproducibility.
The Constant Systems high-pressure cell disruptor has a dead volume of less than 1ml (at least less than 0.1ml) and no minimum sample processing capacity limit.
Constant Systems can process samples with a concentration of up to 30% bacterial concentration, resulting in high concentration of broken sample products, making it convenient for downstream separation and purification operations; Can handle filamentous bacterial cells.
Constant Systems TS, B, and C series optional peristaltic pumps for fully automatic injection and fully enclosed operation, to avoid affecting or being affected by the environment.
The operation and maintenance are simple, and CIP and SIP are very convenient. Specific CIP and SIP designs can be selected, which comply with GMP and FDA standards.
Digital display of operating pressure and sample
temperature, and optional data acquisition package to display and record the
entire disrupting process's operating pressure and sample temperature,
providing useful information for GMP.
Application of Constant Cell Disruptor
The Constant Systems cell fragmentation
instrument is widely used and can be used for cell fragmentation, protein and
DNA extraction, enzyme release, DNA manipulation selective fragmentation,
single-cell separation, tissue fragmentation, etc., such as:
Algae |
disrupting
various collar algae |
Animal cell |
selection fragmentation of chicken sperm cells |
Microbial |
fragmentation and recombination of Escherichia coli
to obtain highly active enzymes; High protein recovery rate achieved by
breaking down Mycobacterium |
Fungi (including yeast) |
Beer yeast releases 99% of the protein, Pichia pastoris
releases 99% of the recombinant protein, and Aspergillus produces high
specific activity enzyme |
Mammalian cells and tissues |
Fragmentation of mammalian cells and tissues by
horse liver cells and endoplasmic reticulum, isolation of single living cells
from bovine spleen and lymph nodes |
insect |
Cytomegalovirus in broken human cell nuclei of
insects |
Plant cells and tissues |
Extract banana pectin from plant cells and tissues, and
extract DNA from strawberry leaves |
virus |
Virus isolation of Toxoplasma gondii membrane
antigen, separation of amniotic membrane cell nucleus and membrane |
Environmental samples |
Extracting DNA from Soil Microorganisms in
Environmental Samples |
Constant Systems is a British company specializing in the production of high-pressure cell and is a member of the Score Group PLC group. Constant Systems has been committed to the development and production of high-pressure disruptors, providing users with high-quality and stable performance disruptors. Through its global marketing network, its products are sold to various parts of the world, including North America, Europe, the Middle East, and Asia, providing high-performance high-pressure disruptors and excellent services to pharmaceutical and biotechnology companies, universities, and research institutions worldwide.