Applitech Sheet-Like Microcarrier Cell Counting Solution

• by Applitech
• 120

Currently, the monitoring of cell status in sheet-like microcarrier cultures typically involves detaching and digesting cells from the carrier using trypsin (crystal violet) for counting. This method has the following drawbacks:

1. Multiple steps and time-consuming process
2. Centrifugation easily causes cell clumping
3. Incomplete trypsin digestion leads to partial cell release
4. Microcarrier interference affects cell counting results (bright field)

Applitech provides the Countleader FL 1000 or FL 100 cell counter, which lyses cell membranes using a lysis reagent (Reagent A+B) to release intracellular nuclei. With fluorescent staining of nuclear DNA, it enables accurate cell counting and monitoring of cell growth status.

1. Simple and fast operation
2. Complete digestion
3. Counting results unaffected by sheet-like carriers
4. Dedicated carrier cell counting program
5. Accurate measurement of cell viability, total cell concentration, and dead cell concentration

Case Study: MRC-5 Cell Culture on Sheet-Like Carriers three sheet-like carriers were taken for lysis and counting, and the average cell count per carrier was compared.

Results:
After lysing with 5 mL of Reagent A for 10 minutes, the obtained cell count was significantly higher—several times greater than that of trypsin digestion. This method provides a more accurate reflection of the actual cell culture conditions in the bioreactor.

Applitech Cell Counter Models

Applitech offers different models of cell counters to meet various sample throughput testing needs.

Countleader FL 2000

1. Dual fluorescence analysis mode with Trypan Blue and AO/PI
2. Built-in carrier cell counting method: "Viability - (AO-PI)"
3. Pre-embedded AO/PI dye in consumables for automatic staining
4. 24-channel system, supporting high-throughput sample analysis
5. All-in-one design with a 12-inch high-definition touchscreen for intuitive operation

Countleader FL 1000

1. Dual fluorescence analysis mode with Trypan Blue and AO/PI
2. Built-in carrier cell counting method: "Viability - (AO-PI)"
3. Cost-effective 8-channel FL8-slides consumables, supporting multi-sample simultaneous analysis
4. Fastest counting time of 25 seconds per sample, significantly improving experimental efficiency
5. All-in-one design with a 12-inch high-definition touchscreen for intuitive operation

Countleader FL 100

1. Compact design, specially tailored for AO/PI dual fluorescence analysis
2. Built-in carrier cell counting method: "Viability - (AO-PI)"
3. Cost-effective 8-channel FL8-slides consumables, supporting multi-sample simultaneous analysis
4. Fastest counting time of 25 seconds per sample, significantly improving experimental efficiency

Lysis Reagent A+B

An alternative to trypsin, reducing issues of incomplete digestion and cell damage, ensuring more accurate cell counting.