Here is the professional English translation of the technical product description for ISHpalette™:

ISHpalette™ Hairpin Amplicons: Simplify Your In Situ Hybridization Experiments and Usher in a New Era of High-Sensitivity Detection!

Are in situ hybridization (ISH) experiments troubling you?

1. Traditional methods are complex, time-consuming, labor-intensive, and often limited by intricate steps like enzymatic treatment.

2. ISHpalette™ is here to save your experiments!

   
   

I. Key Advantages:

1. Multiplex Fluorescence In Situ Hybridization (ISH) Technology

   • Low background noise & high sensitivity

   • Excellent reproducibility & quantitation

   • High quality & cost-effectiveness

2. Superior Compatibility

   • Compatible with immunostaining & double staining

   • Requires no protocol optimization

3. Simple & Fast Operation

   • No enzymatic treatment required; fewer steps make it easy for any lab personnel

   • No need for new equipment

4. Probe Design Service Offered

   
   

II. ISHpalette™ Workflow
Requires no special equipment or reagents. Eliminates the need for proteinase K permeabilization, acetylation, high-temperature treatment, or RNase treatment, significantly simplifying the protocol and accelerating the staining process.

III. What is Hybridization Chain Reaction (HCR)?

1. An enzyme-free signal amplification ISH technology based on hairpin DNA:
   Utilizes the self-assembly and extension of hairpin-structured nucleic acids to mediate a chain reaction of labeled DNA on tissue sections, enabling visualization of target nucleic acids. This technology does not rely on antibodies or enzymatic reactions, offering linear signal amplification and ultra-high signal-to-noise ratio.

   
   

2. Dual-Probe Precision Targeting Mechanism:
   When two probes (both containing HCR initiator sequences) bind to the target, they trigger a cascade amplification reaction of fluorescently labeled hairpin DNA, enabling precise localization of target mRNA and minimizing non-specific reactions.

   
   

IV. Experimental Performance

1. Single-Molecule Detection Sensitivity:

   
   
   Simultaneous detection of Vglut2 (green), Sik3 (red), and Vgat (magenta) mRNA signals on mouse brain sections. Individual mRNA molecules are visualized as distinct punctate spots.

2. Performance Comparison:

   
   
   Traditional HCR vs. ISHpalette™ for simultaneous detection of Penk (top panel), Vglut2 (middle panel), and Nts (bottom panel) mRNA expression in mouse brain sections, with signal intensity comparison. Results clearly demonstrate that ISHpalette™, utilizing short hairpin DNA, offers superior tissue penetration and reaction activity. No proteinase K treatment was used in any experiment.

3. No Enzymatic Treatment Required:

   
   
   Detection of c-Fos protein via immunostaining (left, green) and Vglut2 mRNA via in situ HCR (middle, magenta) on mouse brain sections. In proteinase K-treated samples (top row), weak c-Fos protein signal is detected. In untreated samples (bottom row), both protein and mRNA signals are clearly detected.

   
   

V. Compatible Sample Types

• Paraffin sections

• Frozen sections

• Free-floating sections

• Adherent/Suspension cells

• Whole tissues

  
  

VI. Ordering Information