The Countleader FL 1000 is an image-based cell counter with brightfield and two fluorescence channels, integrated advanced optics, image recognition systems and statistics. In the brightfield (Trypan Blue) and fluorescence (Acridine Orange, AO/ Propidium Iodide, PI) analysis modes, up to 8 samples can achieve accurate viable and dead cell concentration, cell viability, diameter, cell morphology and other information simultaneously.

Easy operation

Sample staining

Simply mix 10 μl of cell sample with 10 μl of the staining solution（AO/PI or Trypan Blue. ）

Loading

Only 15 μl of stained samples is required per well. The advanced FL8-slides can be compatible with multichannel pipettes.

Chamber selection and Press RUN

Put the FL8-slides into the slot of Countleader FL 1000. Selecting the counting Chamber, and Press run to start counting.

Fast and Automated Analysis

FL1000 perform cell counting fast with automatic focus and analysis technology, 8 samples counting in less than 2minutes, the fastest mode is 15sec/ sample for Trypan mode.

Multi-chamber FL8-slideswith unique Code

Each FL8-slides has unique Code for track and the volume of each well has been calibrated to provide high precision cell counting data. The multi-chamber FL8-slides enables high-speed cell counting with accuracy and precision. Within 2 minutes, you will have 8 cell counts and viability measurements, and only a small sample volume is required.

Powerful and Flexible Software

The software is flexible , parameters setting is easy and detection channels can be selected accordingly.

• Larger analysis volume ,Less statistical errors.

• 1 ~ 3 visual fields can be selected for analysis.

• The data can be searched by experiment name, time, and sample ID.

• Separate sample ID facilitate results browsing and searching.

• Dilution Factor is compensated automatically.

• Get cell growth curves to understand the trend of cell growth.

Various forms of results are presented in the software

• Intuitive data with total cell concentration, live and non-viable cell concentration, cell viability, cell diameter and Aggregated cell ratio.
• The data can be represented in histograms, scatter plots and result boxes in the Analysis Manager Plot.

The integrated computer storage space is 2T, can store more than 25000 Samples data.

• The Data can be exported as PDF, Excel, TIFF.
• It is easy to export data quickly with USB3.0.

Accurate results

Excellent linearization of dilution ranges for AO/PI and Trypan blue modes in the FL1000 cell counter.

Count messy samples more accurately with AO/PI fluorescence! With AO/PI model, you can eliminate red blood cells, platelets and debris from your counts using fluorescent nuclear stains AO/PI. Acridine orange (AO) and propidium iodide (PI) are perfect for counting the nucleated cells in samples like PBMCs, patient samples, tumor digests and samples with lots of debris.

Red blood cells are indicated in the bright field+ Fluorescent image (right). The red blood cells and debris are not visible and counted in the fluorescent image (left and middle) detecting cells stained with nuclear staining dye.

Growth curves help to optimize cell culture conditions

The Countleader FL1000 can track and query cell culture parameters, generate density and viability curves of continuous cultured cells, help user understand cell growth status, trends and optimize cell culture processes.

21 CFR part 11 compliant

FL1000 has strict three-level user management and authority, meet the 21 CFR Part 11 requirements of GMP.

• User login with passwords

• User assigned permissions

• Audit trail

• Electronic records and signatures

IQ/OQ/PQ Verification Services

IQ, OQ, PQ (3Q) certified services with a variety of concentration beads and verification documents can be purchased. 3Q operating manuals ensure consistent procedures, generate standardized PDF report documents, fully document sample usage and meet audit trail requirements.

Applications

Various cell samples has been verified in the FL1000, such as CHO、Hek293、PBMC、Car-T and tumor cells. Countleader FL 1000 meets the requirements of high precision in primary cells and cell therapy field and high throughput in CLD and PD department. It is also suitable for cell counting in antibody and vaccine production. Countleader FL 1000 can detect up to 8 samples in less 2 minutes, improves cell counting efficiency greatly!

• Antibody R&D and Production

• Vaccine Production

• PBMC, Car-T in cell therapy

• Stem Cells

• Cancer Cells

• CEF Cells

• and more