Countleader FL 1000  Dual Fluorescent Cell Counter
Countleader FL 1000  Dual Fluorescent Cell Counter
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Countleader FL 1000 Dual Fluorescent Cell Counter

  • AO/PI Dual-Fluorescence Analysis for High Accuracy Fast Analysis
  • Fast Analysis —As Fast as 10 s/Sample
  • Low Sample Volume —Only 15 µL
  • No Cleaning,Calibration,or Maintenance
  • 12"HD Touchscreen Display
  • Description

  • Brand

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The Countleader FL 1000 is an image-based cell analysis system equipped with two fluorescence channels,integrating advanced optical and image recognition technologies.It comes with multiple pre-configured assay modes,including Trypan Blue cell counting,AO/PI dual-fluorescence cell counting,GFP/RFP transfection analysis,cell cycle analysis,and apoptosis analysis.These pre-configured assays significantly simplify and streamline cell analysis workflows in the laboratory.


Simple Workflow with Automated Analysis

FL 1000 enables automated analysis with just staining and sample loading.With only 15 µL of sample,results can be obtained directly from sample loading to analysis.The corresponding analysis results are automatically displayed for each sample.

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• Compatible with Multichannel Pipettes for 24-Sample Analysis


• Fast Analysis —10 s/Sample


• Dedicated QR Codes for Consumables Ensure Accurate Volume Tracking and Reliable Results



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• Disposable &Reusable Consumables


• 200 µm Chamber Depth —Up to 7×Analysis Volume

• Reusable with Dedicated Cleaning Solution —Lower Costs


Powerful & Flexible Software Interface 
User anagement 
The Countleader FL 1000 provides three levels of user access to support GMP and 21 CFR Part  11 compliance, including:
  • Secure User Login
  • Multi-Level User Access
  • Audit Trail
  • Electronic Signature 

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Consumable Parameter Settings 
The Countleader FL 1000 offers flexible and intuitive sample setup: 
  • Easy Consumable Parameter Setup & Customizable Detection Channels
  • Large Analysis Volume with 1–3 Fields of View
  • Individual Sample Naming for Easy Data Tracking
  • Automatic Cell Dilution Factor Compensation

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Data & Analysis 
Quick Search  
Search Results by Experiment Name, Date,or Sample Name
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Multiple Result Views 
  • View Cell Concentration,Viability,Cell Diameter &Aggregation Rate
  • View Detailed Results for Each Sample with the Analysis Manager
  • Countleader FL 1000 provides 3Q validation services,with be

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Data Storage & ExportData Storage &Export

Data Storage & Export
  • 2 TB Built-In Storage for >25,000 Data Records
  • Export Results in PDF,Excel, or TIFF Formats
  • USB 3.0 for Fast &Easy Data Transfer


IQ/OQ/PQ Validation

The Countleader FL 1000 provides 3Q validation services, with beadsat multiple concentrations to meet different cell concentration testing requirements. Validation documentation is provided to support subsequent regulatory submissions.


GFP/RFP Transfection Efficiency

Transfection efficiency refers to the percentage of cells that successfully express the introduced GFP/RFP gene. The FL 1000 provides a fast and simple method for GFP/RFP transfection analysis. By detecting the fluorescence emitted by GFP/RFP under specific excitation wavelengths, it delivers instant results, including GFP/RFP-positive cell percentage,concentration, and cell diameter.

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Cell Cycle

The FL 1000 enables cell cycle analysis by determining the proportion of cells in the G0/G1,S,and G2/M phases. It helps assess cell proliferation rates and evaluate whether drug treatments inhibit cell proliferation.

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Red:G0/G1 Phase;Yellow:S Phase;Green:G2/M Phase;Blue:Cells Outside the Cell Cycle

Cell Aggregates are excluded from analysis to ensure accurate single-cell data and reliable cell cycle results.


Apoptosis 
Apoptosis is a form of programmed cell death characterized by a series of biochemical and morphological changes. The FL 1000 uses Annexin V-FITC and PI staining to classify cells into different stages of apoptosis, providing rapid, automated analysis with immediate results.
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