• Auto-focus with the classic trypan blue staining method
• Rapid analysis: 8 samples in just 90 seconds!
• Minimal sample volume required: only 15 μL
• No cleaning, calibration, or maintenance required
• Compliant with 21 CFR Part 11
The Countleader BF 100 utilizes the trypan blue exclusion method, integrating advanced optical systems and intelligent image recognition technology. It can simultaneously analyze 8 samples with precision, providing accurate information on total cell count, concentration, viability, diameter, aggregation ratio, and morphology within 1–2 minutes.
Simple Operation Workflow
Sample staining
Simply mix 10 µl of cell samples with 10 µl of the Trypan Blue staining solution.
Load and Press RUN
Only 15 µl of stained sample is required. The advanced FL8 slides are compatible with multichannel pipettes. Insert the FL8 slides into the Countleader BF100 slot and press RUN to start the counting process.
Fast and Automated Analysis
The BF100 performs cell counting quickly with automatic focus and analysis technology. It can count 8 samples in under 2 minutes.
Multi-Chamber FL8-Slides with Unique Code
Each FL8-Slide is equipped with a unique QR code to accurately record and calibrate the analysis volume of each well, ensuring more precise results. With minimal sample volume, high-precision rapid cell counting for 8 samples can be completed in just 2 minutes.
Powerful and Flexible Software
Easy Software Operation
• Detection channels can be selected accordingly.
• Larger analysis volume reduces statistical errors; choose from 1–3 visual fields for analysis.
• Separate sample IDs enable easy result browsing and searchability.
• Automatic compensation for dilution factor ensures accurate results.
Data & Analysis
The data can be searched by experiment name,time, and sample lD.
• Results are presented in various formats, with intuitive data showing total cell concentration, viable and dead cell concentration, cell viability, cell diame ter, and aggregated cell ratio
• Cell growth curves provide insight into cell growth trends
• Data can be exported in PDF, Excel, or TIFF formats for further analysis and reporting
User Management
The BF100 features a strict three-level user management system with defined permissions, fully compliant with 21 CFR Part 11 for GMP regulations.
• Access protected by user-specific passwords
• Customizable user permissions for enhanced control
• Audit Trail
• Electronic Records & Signatures
IQ/OQ/PQ Verification Services
IQ, OQ, and PQ (3Q) certified services are available, with a variety of concentration beads and accompanying verification documents for purchase. The 3Q operating manuals ensure consistent procedures, generate standardized PDF reports, fully document sample usage, and meet audit trail requirements.
Application
Countleader Cell Counter Series