The Countleader FL 100 is an image-based fluorescence cell counter equipped with two fluorescence channels and an advanced integrated optical and image recognition system. Using AO/PI fluorescent staining, it can accurately analyze up to eight samples simultaneously. By combining fluorescence imag ing with statistical analysis, it delivers precise information on cell concentration, viability, and morphology.

Streamlined Workflow with Fully Automated Analysis

Cell Staining & Sample Preparation

Mix cells and AO/PI dye at a 1:1 ratio thoroughly to ensure uniform staining

Sample Loading

• Uses dedicated 8-channel FL8 slides

• Requires only 15 μL per well

• Compatible with multichannel pipettes for simplified handling

Rapid Analysis

• Select the appropriate sample well and set parameters to start  

• Auto-focusing—no manual adjustment needed  

• Analyze 8 samples in 3–4 minutes; as fast as 25 seconds per sample! 

Powerful and Flexible Software Interface 

Consumable & Sample Settings

• Easily set sample parameters and customize detection channels

• Large sample counts reduce statistical error; select 1–3 fields of view for analysis

• Individually name each sample for easy data retrieval

• Single-well mode supports multiple analysis methods simultane ously

• Automatically compensates for cell dilution factor

Data & Analysis

Quick Search

Effortlessly locate results by experiment name, date, or sample name.

Comprehensive Data Display

• Viability

• Live cell concentration

• Total cell concentration

• Dead cell concentration

• Cell diameter

• Cell roundness

• Aggregation ratio

• Growth curve

• Pseudo-flow scatter plot

• Fluorescent cell images

• Cell diameter distribution graph

Multi-Format Export

Results can be exported directly in PDF, Excel, or TIFF formats for convenient viewing and archiving.

User Management 

The Countleader FL 100 supports a three-level user login system in compliance with GMP and 21 CFR Part 11, offering:  

• Secure user login 

• Multi-level user permissions 

• Audit trail  

• Electronic signature 

3Q Verification (IQ/OQ/PQ) 

The Countleader FL 100 offers full 3Q validation services, including IQ (Installation Qualification), OQ (Operational Qualification), and PQ (Performance Qualification). Validation beads at various concentra tions are available to support different cell concentra tion testing needs. Complete validation documenta tion is provided to facilitate regulatory submission and compliance.

Applications 

CAR-T Therapy

AO/PI staining targets cellular nuclear DNA, providing accurate and reliable counts without interference from anucleate compo nents such as red blood cells, platelets, debris, bubbles, or magnetic beads. It is ideal for counting CAR-T cells, PBMCs, low-puri ty cells, and irregular or stressed cells—no need to adjust parameters based on cell morphology or diameter, reducing potential counting errors.

Microcarrier Cell Counting 

Applitech offers a gentle and effective lysis solution (Reagent A + B) that chemically disrupts the cell membrane and other struc tures to release intracellular contents. When used with the Countleader FL 100 and its dedicated analysis method (Viability-(A and B)), accurate cell counting on microcarriers is achieved.

• Gentle lysis reduces issues caused by incomplete trypsin digestion and cell damage

• AO/PI fluorescence staining enables accurate cell counting without interference from microcarriers

• Simple one-step procedure provides viability, live cell concentration, and total cell concentration

Countleader Cell Counter Series