Countleader FL 2000 cell counter is a high-throughput automated system for cell counting analysis using the FL24-plate with pre-coated dyes and analyzing 24 samples in 13 minutes to meet the needs of customers for multi-samples analysis.

Key Features & Benefits

• Automatic staining technology

• Analyze 24 samples in less than 13 minutes

Total cell, live cell, dead cell count

Viability

Cell diameter

Growth curve

% of cells in clumps

• Only 30 μL of cell sample required

• Calibration, maintenance and service free

• Customizable PDF reports

• 21 CFR part11 compliant

Product Details

Automatic staining technology

The FL24-plate is designed to be coated with AO/PI dyes in the microfluidic channel, once the cell samples are loaded, the cell samples are automatically aspirated and flow through the microfluidic channel, stained by the coated AO/PI automatically and finally pushed into the detection chamber. In the chamber, the stained samples are excited and analyzed, all the cell counting data are displayed in the software.

The FL24-plate estimates the error by manual, resulting in accurate data, makes your work more efficient and convenient!

The inside of the microfluidic channel of FL24-Plate is coated with Acridine orange and PI, staining the entire population of cells and non-viable cells respectively. The volume of each FL24-Plate has been calibrated to provide high precision cell counting data.

Easy operation process

Sample Loading directly!

Loading 30μL of sample into the FL24-plate plate with Multi-channel pipette, no pretreatment of the samples is required.

Load and press “Run”

The FL24-plate with samples is loaded into the FL2000 and Press Run.

Automatic staining and analysis

Samples are stained automatically as the samples flow through the microfluidic channel coated with AO/PI. The analysis process is fully automated, accurate cell density, viability and fluorescence images of 24 cell samples are presented in less than 13 minutes.

Powerful and flexible software

The software is powerful, friendly and flexible, detection channels can be selected according to your sample numbers.

• Typan blue and AO/PI modes are available

• Larger analysis volume, Less statistical errors

• The data can be searched by experiment name, time, and sample ID

• Separate sample ID facilitate results browsing and searching

• Dilution Factor is compensated automatically

• Get cell growth curves to understand the trend of cell growth

Accurate Data

Automatic staining and analysis make the results accurate, without the error from the staining of samples by manual. With 1.5X magnification, the area of analysis of field of view is about 4.7 * 3.1m², which is almost 12 times of that in the Hemocytometer method. The number of cells detected in a single sample can reach tens of thousands, effectively reducing counting errors.

Intuitive and accurate data with total cell concentration, live and non-viable cell concentration, cell viability, cell diameter and Aggregated cell ratio can be obtained fastly.

Multiple results are represented in the software. The data can be represented in histograms, fluorescent scatter plots and result boxes in the Analysis Manager Plot.

All the data can be exported in PDF, Excel, TIFF format.

Polygons and markers can be drawn in the plots and are used for gating and counting of subpopulations. The percentage of cells with different diameter can be achieved to know the cell status such as stem cell differentiation, virus infection efficiency.

The software is with powerful functions, cells can be gated to check whether the cells are correctly recognized and counted, achieving accurate cell counting.

Tracking and detecting information of continuously cultured cell samples can be plotted as a cell growth curve.

√Concentration curve

√Viability curve

It is easy to track the cell growth state and trends of cells, which is conducive to the optimization of cell culture technology.

21 CFR Part 11 Compliant

Countleader FL 2000 has strict 3-levels user management and authority, meet the 21 CFR Part 11 requirements of GMP. Modules have the following functions:

• User login with passwords

• User assigned permissions

• Audit trail

• Log files

• Electronic records and signatures

IQ/OQ/PQ Verification

Countleader FL 2000 provides 3Q verification service with a variety of concentration beads and verification documents to facilitate the later declaration work.

Compatible with various consumables

In addition to the FL24-plate coated with AO/PI dyes, Countleader FL2000 is compatible with other different consumables, empty 24-channel FL24-plate without coated dye and 8-channel FL8-slides, manual staining can also be used for Trypan blue stain counting in Countleader FL 2000.

Product Applications

In the cell line development and process development, many samples are required for cell counting, the daily cell count workload is large. Countleader FL 2000 provide the solution with high throughput, low sample consumption, fast and automatic staining and analysis. Countleader FL 2000 can detect 24 samples in less 13 minutes, improves cell counting efficiency greatly!

• CLD department in Antibody and CGT field

• PBMC, Car-T in cell therapy

• Stem Cells

• Cancer Cells

• and more