The Countleader® FL 2000 is a fully automated, high-throughput cell counter designed to meet the demands of multi-sample analysis. Using the patented FL24-Plate with microfluidic channels, the system supports automatic staining. It enables dual-fluorescence staining (AO/PI) analysis for 24 samples in just 17 minutes (12 minutes for trypan blue staining).

Automatic Staining Technology

FL24-Plate-A features a unique design with pre-embedded dyes. The AO/PI dyes are preloaded in the microfluidic chan nels. Simply add 40 µL of cell sample per well, and as the cells flow through the microfluidic channels, staining and analysis are performed automatically. This is ideal for high-precision counting of a large number of samples that need to be measured daily."

FL24-Plate-B is an empty 24-channel consumable plate. Add 20 µL of cell sample and 20 µL of trypan blue dye per well. The sample is automatically mixed and stained as it flows through the microfluidic channels. This allows for the analysis of 24 samples in just 12 minutes, making it perfect for cell counting during process optimization phases with a high number of daily samples

• The patented design of the microfluidic channels eliminates errors caused by manual staining, ensuring precise and reliable counting results while making your work more efficient! The disposable FL24-Plate keeps all samples confined within the consumable, with no flow paths in the machine. Therefore no cleaning or maintenance is required after use.

• Each FL24-Plate is equipped with a unique QR code to accurately record and calibrate the analysis volume of each well, ensuring even greater precision in results.

Simple Operation Workflow

Sample Loading

AO/PI Mode:Only 40 µL of cell sample per well is required. Compatible with multichannel pipettes, no pre-staining needed.

Trypan Blue Mode: Only 20 µL of cell sample and 20 µL of trypan blue dye per well, with no manual staining required.

Loading the Plate

Place the FL24-Plate into the designated slot of the Countleader FL 2000, select the wells to be counted, and start the analysis.

Automatic Staining and Analysis

Samples are stained automatically as they flow through the microfluidic channel. The fully auto mated system quickly delivers cell density, viabili ty, and both bright-field and fluorescence images for all 24 samples with precision.

• Viable Cell Density
• Dead Cell Density
• Total Cell Density
• Cell Diameter
• Cell Aggregation Ratio
• Cell Fluorescence Images
• Cell Fluorescence Intensity Distribution Charts
• Cell Diameter Distribution Charts

Powerful and Flexible Software

The software features a flexible and user-friendly system interface with customizable detection channels.

• Typan blue and AO/PI modes are available

• Larger analysis volume reduces statistical errors

• The data can be searched by experiment name, time, and sample ID

• Separate sample ID facilitate results browsing and searching

• Dilution Factor is compensated automatically

• Growth curves aid in understanding and optimizing cell culture conditions

Accurate Data

The Countleader FL2000 ensures precise results through its automatic staining and analysis process, eliminating errors caused by manual staining. With 1.5X magnification, the analysis area per field of view measures approxi mately 4.7 × 3.1 mm²—nearly 12 times larger than the traditional Hemocytometer method. Tens of thousands of cells can be detected in a single sample, significantly reducing counting errors and ensuring reliable results.

CHO cell concentration gradient plot

• Quickly obtain clear and accurate information on total cell density, viable cell density, dead cell density, cell viability, cell diameter, and cell aggregation ratio.

• Use the Analysis Manager to view detailed data for each sample.

• Data can be exported in PDF, Excel, TIFF format.

Polygons and markers can be drawn directly on plots for precise gating and counting of subpopulations. The software also provides the percentage of cells with varying diameters, offering insights into cell status, such as stem cell differentiation and viral infection efficiency.

The software offers robust functionality to ensure accurate cell counting. Cells can be gated to verify proper recognition and counting, guaranteeing precise results.

AO/PI fluorescence image A: original image; B: Magnified image, Gated cells are checked and recognized correctly

Continuously cultured cell samples can be moni tored and visualized as cell growth curves, including:

Density curve

Viability curve

It is easy to track the cell growth state and trends of cells, which is conducive to the optimization of cell culture technology.

Compliant with 21 CFR Part 11

The Countleader® Series offers three levels of login permissions, meeting GMP requirements under 21 CFR Part 11. Key features include:

• Secure user login

• Multi-level user permissions

• Audit trails

• Log files

• Electronic signatures

IQ/OQ/PQ Verification

IQ, OQ, and PQ (3Q) certified services are available, with a variety of concentration beads and accompanying verification documents for purchase. The 3Q operating manuals ensure consistent procedures, generate standard ized PDF reports, fully document sample usage, and meet audit trail requirements.

Compatible with various consumables

Applications

In CLD and PD departments, where a high volume of cell samples requires counting, the workload can be overwhelm ing. The Countleader FL2000 provides a high-throughput solution with low sample consumption, fast processing, and automated staining and analysis.

• CLD department in Antibody and CGT field

• PBMC, Car-T in cell therapy

• Stem Cells

• Cancer Cells

• and more